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Biosynthesis, characterization of silver nano particles from Agaricus bisporus (Mushroom) and their antimicrobial activity against foodborne bacterial pathogens

Article scientifique 2023 Anglais

Résumé

Abstract Biosynthesized silver nanoparticles (AgNPs) attracted broad interest in the food sector due to their excellent antimicrobial activities as a new alternative to harmful chemical disinfectants. This work aims to study characterization parameters, antimicrobial and toxicological characteristics of the biosynthesized silver nanoparticles from Agaricus bisporus (Mushroom) aqueous extract. Mushroom extract was treated with silver nitrate and incubated for 24h until its color was turned brown. AgNPs characterization studies were estimated for their stability, their characteristics using UV and visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HR-TEM), Dynamic light scattering (DLS) and energy dispersive X-ray spectroscopy (XRD). All synthesized particles had the maximum absorption at 456nm at 1.5 Surface plasmon resonance (SPR), indicating the biosynthesis of nano silver particles. In addition. AgNPs from A. bisporus (Mushroom) exhibited broad antibacterial action against eight foodborne bacterial strains. Among all the tested bacterial strains, P. aeruginosa ATCC 27853 was the superlative sensitive strain with inhibition zone of 48mm, while IZD for K. pneumonia ATCC 700603 and B. cereus ATCC 11778 were the most resistant strains with IZD of 20mm. According to the MIC/MBC ratio, the silver cations released by AgNPs had strong bactericidal effects against both G+ ve and G− ve bacteria. Cytotoxicity test against normal human skin fibroblast indicated that silver nano silver particles from the mushroom extract were safe, exhibiting cell viability reached 98.2%. Therefore, AgNPs could be a good alternative source to inhibit the biofilm formation in the food industry sector.

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Amr, M., Abd-Elhalim, B., Ismail, R., Aboubakr, A., Wael, R., Yasser, M., Hemdan, B., El-Sayed, S., Abu-Hussien, S., Bakry, A., Ebeed, N., Elhariry, H., Galal, A. (2023). Biosynthesis, characterization of silver nano particles from Agaricus bisporus (Mushroom) and their antimicrobial activity against foodborne bacterial pathogens. https://doi.org/10.21203/rs.3.rs-3077554/v1

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Auteur(s)

Maryam Amr Basma T. Abd-Elhalim Radwa Ismail Asmaa Aboubakr Rahma Wael Mariam Yasser Bahaa A. Hemdan Salwa Mahmoud El-Sayed Samah H. Abu-Hussien Ashraf Bakry Naglaa M. Ebeed Hesham Mohsen Elhariry A. Galal

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