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Antiviral and protective effect of small interfering RNAs against Rift Valley Fever Virus In Vitro

Article scientifique 2022 Anglais

Résumé

Abstract Rift Valley Fever Virus (RVFV) is an arbovirus, zoonotic pathogen that repeatedly reinvigorates with a possible threat across geographic borders. There are no authorized drugs for RVFV. RNA interference gene silencing pathway is a highly conserved mechanism. Specific and different small interfering RNAs (siRNA) have been designed against RVFV Nucleoprotein genes by different bioinformatics tools to investigate their antiviral potentialities in the Vero cell line. Real-time PCR besides Endpoint assay was used to determine the silencing activity of siRNA and reduction in gene expression as all designed siRNAs abrogate mRNA replication by more than 90% by RT-PCR and significant inhibition in RVFV replication in cell line when used as antiviral as well prophylactic therapeutics. Western blot analysis was utilized for protein abundance detection after 48 hours. Consequently, specific siRNA can inhibit RVFV replication in a cell-based assay, introducing novel and promising RVFV epidemics and epizootics therapeutics.

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Ahmed, E., Boseila, A., Hanora, A., Solyman, S. (2022). Antiviral and protective effect of small interfering RNAs against Rift Valley Fever Virus In Vitro. https://doi.org/10.21203/rs.3.rs-1576028/v1

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Auteur(s)

Engy M Ahmed Abeer A. Boseila Amro Hanora Samar M. Solyman

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