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The role of microRNA expression in malignant transformation of pleomorphic adenoma: comparing carcinoma ex pleomorphic adenoma vs pleomorphic adenoma

Article scientifique 2022 Anglais

Résumé

Abstract Purpose Carcinoma ex pleomorphic adenoma (CXPA) is a rare malignant tumor which transformed from benign PA. Here we identify the characteristic miRNAs from both the PA (CPA) and carcinomatous (CA) portions of CXPA in an effort to understand their malignant transformation and then compare their expression in CPA and benign PA (BPA) samples to identify candidate miRNAs for their differential diagnosis. Methods We selected 13 CXPA FFPE and 16 BPA samples and then separated the CPA and CA portions of the CXPA FFPE tissues before completing total RNA extraction and miRNA profiling for each group. Results Downstream evaluation of these miRNAs identified 13 transcripts that were differentially expressed between the CPA and CA portions of CXPA. A total of eight of these miRNAs were up-regulated and five down-regulated in CA, where the up-regulated miRNAs were related to cancer progression and the down-regulated miRNAs were associated with tumor suppression. In addition, seven miRNAs were significantly up-regulated in CPA when compared to BPA, even though they present with the same histopathology. These transcripts also all demonstrated a clear relationship with cancer when evaluated using gene ontology and KEGG pathway tools, with almost all of these transcripts interacting with TP53.Conclusion We identified several differentially expressed miRNAs in the CPA and CA portions of CXPA, and confirmed that they were closely associated with TP53 and various cancer-related pathways using target gene and pathway analyses. In addition, we also identified several differentially expressed miRNAs in CPA and BPA, which are also associated with carcinogenesis and may serve as potential biomarkers for differential diagnosis.

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Kim, H., Eun, S., Jeong, W., Ahn, S., Bae, Y., Lee, J., Kim, H. (2022). The role of microRNA expression in malignant transformation of pleomorphic adenoma: comparing carcinoma ex pleomorphic adenoma vs pleomorphic adenoma. https://doi.org/10.21203/rs.3.rs-1504827/v1

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Auteur(s)

Hyojin Kim Shin Eun Woo‐Jin Jeong Soon‐Hyun Ahn Yun Jung Bae Joong Seob Lee Heejin Kim

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