Accès ouvert

Role of microRNA-183 based theranostics through targeting TPM1 in bladder cancer

Article scientifique 2025 Anglais

Résumé

BACKGROUND: MicroRNA-183 (miR-183-5p), a noncoding RNA, is upregulated in bladder carcinoma (BC). Although it has been implicated in oncogenesis, its precise regulatory effects and biological functions remain unclear. Tropomyosin-1 (TPM1) was shown to be downregulated in solid tumors and was previously identified as a novel tumor suppressor gene. OBJECTIVES: Our study focuses on the prognostic, diagnostic, and therapeutic potential of miR-183-5p in bladder carcinoma and assess TPM1 gene targets and their modulatory functions. METHODS: Urine cytology, cystectomy and transurethral resection (TUR) biopsies from 148 BC patients were collected. TPM1 protein and miR-183-5p expressions were assessed through immunohistochemistry (IHC) and real-time PCR respectively. In vitro assay investigated the effect of miR-183-5p on TPM1mRNA in bladder carcinoma cell lines, then confirmed by comparing miR-183-5p mimics in non-cancerous and urothelial carcinoma cell lines. Concomitant TPM1 gene expression was examined, and the theranostic miR-183-5p potential was evaluated. RESULTS: Upregulation of miR-183-5p expression in BC tissue biopsies and urine cytologies was noted, contrasting the downregulation of TPM1 protein expression in the high-grade, high-stage, lymph node metastatic BC tissues (in comparison to non-cancerous, low-grade, low-stage non-lymph node metastasizing BCs). Moreover, the miR-183-5p oncogenic influence was responsive in targeting TPM1 gene at 3'UTR region, and miR‑183‑5p.1 restricted TPM1 expression in T24 cells. CONCLUSION: This study provides the first illustration of the miR-183-5p-TPM1 axis in bladder carcinoma, supporting the theranostic role of miR-183-5p as an onco-miR in BC progression, diagnosis, and prognostication.

Citer ce document

Mamdouh, S., Aboushousha, T., Hemida, E., El-Araby, R., Elesaily, K., Hammad, G., Magdy, M. (2025). Role of microRNA-183 based theranostics through targeting TPM1 in bladder cancer. https://doi.org/10.1007/s12672-025-03829-w

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Voir l'article sur le site de la revue

Auteur(s)

Samah Mamdouh Tarek Aboushousha Eman H. A. Hemida Rady E. El-Araby Khaled Elesaily Gehan Hammad Mona Magdy

Statistiques

Consultations : 1

Téléchargements : 0