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6th International Symposium on Molecular Allergology (ISMA)

Article scientifique 2016 Anglais

Résumé

Background: Delayed food anaphylaxis upon consumption of red meat is attributed to specific IgE-antibodies directed to galactose--1,3-galactose (-Gal). Anaphylactic reactions may occur after ingestion of meat from different mammals, mainly beef and pork, but reactions to lamb, rabbit or horse have also been reported. In particular, pork kidney has been shown to trigger symptoms that were more severe and occurred within a shorter delay. The objective of the present study was the identification and characterization of pork kidney proteins carrying -Gal carbohydrates and mediating delayed allergic reactions through specific IgE to -Gal. Materials and methods: A cohort of 59 patients with specific IgE to -Gal was screened by immunoblot for IgE-reactive proteins in pork kidney extract. Proteins were purified by affinity chromatography and identified by Edman sequencing and peptide mass fingerprinting. Isolated proteins were used in immunoassays using patient sera and -Gal specific antibodies. Allergenicity was assayed in basophil activation and skin prick test. Results: Multiple IgE-binding proteins were detected in protein extracts of pork kidney by immunoblot using patient sera and an anti--Gal antibody. Reactive bands were located in the high molecular weight range of 100 to 200 kDa. Two major IgE-binding proteins were identified as porcine angiotensin I converting enzyme (ACE I) and aminopeptidase N (AP-N). IgE-binding to both proteins was lost by periodate treatment, resulting in oxidation of carbohydrates. Addition of -Gal inhibited IgE-reactivity to both peptidases. Allergenicity was confirmed by activation of patient basophils and positive skin prick tests. Conclusions: Two IgE-reactive cell membrane peptidases carrying -Gal epitopes were identified in pork kidney, a tissue which is known as potent inducer of red meat-induced anaphylaxis. Allergenicity and clinical relevance of these proteins were confirmed in patients with delayed anaphylaxis to red meat by skin prick test and basophil activation.

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Hilger, C., Swiontek, K., Fischer, J., Hentges, F., Lehners, C., Morisset, M., Eberlein, B., Biedermann, T., Ollert, M., Wildner, S., Stemeseder, T., Freier, R., Briza, P., Lang, R., Batanero, E., Villalba, M., Lidholm, J., Hawranek, T., Ferreira, F., Brandstetter, H., Gadermaier, G., Moingeon, P., Groeme, R., Bouley, J., Bordas, V., Mignon, M., Bussières, L., Lautrette, A., Mascarell, L., Lombardi, V., Baron‐Bodo, V., Chabre, H., Batard, T., Nony, E., Lima, K., Watanabe, A., Figo, D., Santos‐Pinto, J., Palma, M., Castro, F., Kalil, J., Wohlschlager, T., Santos, K., Faber, M., Gasse, A., Sabato, V., Hagendorens, M., Bridts, C., Clerck, L., Díaz‐Perales, A., Ebo, D., Zavadáková, P., Buchwalder, A., Rebeaud, F., Märki, I., Gepp, B., Lengger, N., Möbs, C., Pfützner, W., Radauer, C., Bohle, B., Galvão, C., Schwager, C., Kull, S., Schocker, F., Behrends, J., Becker, W., Jappe, U., Mastrorilli, C., Tripodi, S., Caffarelli, C., Asero, R., Dondi, A., Ricci, G., Dascola, C., Calamelli, E., Businco, A., Bianchi, A., Frediani, T., Verga, C., Iacono, I., Peroni, D., Pingitore, G., Bernardini, R., Matricardi, P., Hofer, H., Asam, C., Hauser, M., Himly, M., Ebner, C., Lemoine, P., Jain, K., Abiteboul, K., Arvidsson, M., Rak, S., Mota, I., Garcia, F., Gáspár, A. (2016). 6th International Symposium on Molecular Allergology (ISMA). https://doi.org/10.1186/s13601-016-0123-x

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Auteur(s)

Christiane Hilger Kyra Swiontek Jörg Fischer François Hentges Christiane Lehners M. Morisset Bernadette Eberlein Tilo Biedermann Markus Ollert Sabrina Wildner Teresa Stemeseder Regina Freier Peter Briza Roland Lang Eva Batanero Mayte Villalba Jonas Lidholm Thomas Hawranek Fátima Ferreira Hans Brandstetter Gabriele Gadermaier Philippe Moingeon Rachel Groeme Julien Bouley Véronique Bordas Maxime Le Mignon Lætitia Bussières Aurélie Lautrette Laurent Mascarell Vincent Lombardi Véronique Baron‐Bodo Henri Chabre Thierry Batard Emmanuel Nony Karine De Amicis Lima Alexandra Sayuri Watanabe Daniele Danella Figo José Roberto Aparecido dos Santos‐Pinto Mário Sérgio Palma Fábio Fernandes Morato Castro Jorge Kalil Therese Wohlschlager Keity Souza Santos Margaretha A. Faber Athina L. Van Gasse Vito Sabato Margo M. Hagendorens Chris H. Bridts Luc S. De Clerck Araceli Díaz‐Perales Didier G. Ebo Petra Zavadáková Aurélie Buchwalder Fabien Rebeaud Iwan Märki Barbara Gepp Nina Lengger Christian Möbs Wolfgang Pfützner Christian Radauer Barbara Bohle Clóvis Eduardo Santos Galvão Christian Schwager Skadi Kull Frauke Schocker Jochen Behrends Wolf‐Meinhard Becker Uta Jappe Carla Mastrorilli Salvatore Tripodi Carlo Caffarelli Riccardo Asero Arianna Dondi Giampaolo Ricci Carlotta Povesi Dascola Elisabetta Calamelli Andrea Di Rienzo Businco Annamaria Bianchi T. Frediani Carmen Verga Iride Dello Iacono Diego Peroni Giuseppe Pingitore Roberto Bernardini Paolo Maria Matricardi Heidi Hofer Claudia Asam Michael Hauser Martin Himly Christof Ebner P. Lemoine K. Jain Kathy Abiteboul Monica Arvidsson S. Rak Inês Mota Filipe Benito Garcia Angéla Gáspár

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