The Inhibitory Effect of Haloxylon, Cichorium spp and Capparis spp on Cercospora leaf spot disease on sugar beet in vitro and vivo

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Abstract Sugar beet (Beta vulgaris L.) is one of the most important sugar crops in the world, which considered the second important sugar crop in Egypt after sugar cane. In Egypt, sugar beet is attack by Cercospora leaf spot disease caused by pathogenCercospora beticolafungal, which caused losses can reach to 40% of yield. In the present study, three plant extracts of Flower ofHaloxylon spp,Cichorium spp, and the leaf ofCapparis spp were used.Different concentration of the three plant extracts (10%,20%,30%,35% and 40%) previously were used in experimentin vitroto determine Antifungal activity on the linear growth ofCercospora beticola .Whilein vivo, the concentration which recorded highest effect againstCercospora beticola in vivowere tested.In vivothe experiment conducted in two treatments, the first one sprayed sugar beet plants by plant extracts before the inoculation and the second treatment sprayed plant extracts after the inoculation. Experimental result show under laboratory, all tested plant extracts at concentrations (30,35 and 40%) showed highly effective on mycelial growth ofCercospora beticolaand inhibited fungal growth compared to the control treatment and (10 and 20%) concentration. Whilein vivoresult show that the treated sugar beet plants with plant extracts before inoculated then byCercospora beticolagive the best result for reduction the disease severity of CLS disease comparing with the control .In addition to result revealed that the treated sugar beet plants with tested extracts after inoculated byCercospora beticolagave the best result for value total of chlorophyll a and chlorophyll b which recorded the highest value comparing with the control treatment.

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