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Environmental sampling using Moore swabs v2

Article scientifique 2025 Autre

Résumé

A protocol for the collection of environmental samples (sewage and wastewater) using Moore swabs and subsequent laboratory processing up to DNA extraction. This is part of a set of protocols for environmental surveillance of Salmonella Typhi. Bacteria in the wastewater are trapped in the Moore swab. Trapped bacteria are enriched during incubation in Universal Pre-Enrichment broth. The broth is then filtered under a vacuum to capture the bacteria on filter discs ready for DNA extraction. This protocol is adapted from the Moore swab protocol described in Liu et al (2021).

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Rigby, J., Troman, C., Abraham, D., Mahindroo, J., John, J., Mohan, V., Srinivasan, R., Zhou, N., Nair, S., Shaw, A., Elviss, N., Feasey, N., Meschke, J., Kang, G., Grassly, N. (2025). Environmental sampling using Moore swabs v2. https://doi.org/10.17504/protocols.io.6qpvrd98pgmk/v2

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Auteur(s)

Jonathan Rigby Catherine Troman Dilip Abraham Jaspreet Mahindroo Jacob John Venkata Raghava Mohan Rajan Srinivasan Nicolette Zhou Satheesh Nair Alex Shaw Nicola Elviss Nicholas Feasey John Scott Meschke Gagandeep Kang Nick Grassly

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