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Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System

Article scientifique 2017 Anglais

Résumé

The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88-100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.

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Khalil, K., El-Ayat, M., Daghash, S., Elaswad, A., Miller, M., Abdelrahman, H., Ye, Z., Odin, R., Drescher, D., Vo, K., Gosh, K., Bugg, W., Robinson, D., Dunham, R. (2017). Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System. https://doi.org/10.1038/s41598-017-07223-7

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Auteur(s)

Karim Khalil Medhat A. El-Ayat Samer Mohamed Daghash Ahmed Elaswad Michael E. Miller Hisham A. Abdelrahman Zhi Ye Ramjie Odin David Drescher Khoi Vo Kamal Gosh William S. Bugg Dalton Robinson Rex A. Dunham

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