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Production and Partial Purification of Pectinases Produced by Three Novel Cladosporium Species Isolated from Egypt

Article scientifique 2022 Anglais

Résumé

Abstract Based on phenotypic characters and sequencing of the internal transcribed spacers (ITS), 28S Large Subunit (LSU) and partial actin (ACT), three novel species of Cladosporium were described in this study as C. parasphaerospermum, C. chlamydosporum, and C. compactisporum. Activities of pectinases produced by the three strains were 5700, 3720, and 3480 U mg− 1 enzymes, at the optimum pH (6.0, 7.0, and 8.0) and temperature (35, 30, and 35 ºC), respectively. The ions potassium and manganese had a strong activating impact on the activity of C. compactisporum pectinase (124.4 and 172%, respectively). When tested under optimal conditions, EDTA and the other metal ions inhibited the activity of the pectinases in varied degrees. The strongest inhibitory effects of C. parasphaerospermum, C. chlamydosporum, and C. compactisporum pectinases were with Ni+ 2, Zn+ 2, and EDTA, respectively. Cladosporium species have a long history of biotechnological uses, and here we add another: pectinase makers. We offer three unique suppliers of low-temperature active fungal pectinases for biotechnological applications such as the management of Pectinacious wastes in the juice industry.

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Moharram, A., Zohri, A., Hesham, A., Maher, M., Al-Bedak, O. (2022). Production and Partial Purification of Pectinases Produced by Three Novel Cladosporium Species Isolated from Egypt. https://doi.org/10.21203/rs.3.rs-1291889/v1

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Auteur(s)

A. M. Moharram A. A. Zohri Abd El‐Latif Hesham Mohamed Al-Amin Maher Osama A.M. Al-Bedak

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