Reviewing Stimulus and System-Specific Differences in Expression Profiles of Methanogenesis Optimized Anaerobic Digestion Transcriptomes

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Abstract Anaerobic digestion is a crucial process in wastewater treatment, renowned for its sustainable biogas production capabilities and simultaneous reduction of environmental pollution. However, dysregulation of vital biological processes and pathways can lead to reduced efficiency and suboptimal biogas output. This study aimed to investigate gene expression patterns in anaerobic digestion and identify potential therapeutic targets using RNA sequencing data from NCBI's Sequence Read Archive (SRA) database. By creating a reference genome that was 86.4% complete we were able to assemble transcripts and follow expression patterns of the different sample groups that were in question. We identified pathways for homeostasis and cellular transport to be the most important in full scale anaerobic digesters and further elaborated on specific reactions and gene expression in this context. By analyzing the homolog signatures with regard to oxidative phosphorylation gene sets, we were able to characterize the heterogenic transcriptome to be absolutely prokaryotic. We discovered variants of highly conserved alleles for electron cycling by the heterodisulfide reductase complex, methylation and demethylation by methyl-transferases, and oxidation of active coenzyme A and B by methyl coenzyme reductase.

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