Optimized formulation of a three-component extract mixture from Moroccan Crocus sativus L. (Stigmas, leaves, and Tepals) for enhanced antioxidant activity

Résumé

Abstract This study evaluated the antioxidant potential of a three-component mixture derived from Crocus sativus L. stigmas, leaves, and tepals, aiming to valorize these underutilized by-products. Hydroethanolic extracts were chemically profiled using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD), identifying major bioactive compounds including crocin (11.04%), rutin (22.32%), and ellagic acid (62.47%). Antioxidant capacity was assessed using DPPH and ABTS assays, yielding IC50 values ranging from 88.67 ± 0.83 µg/mL to 291.41 ± 0.91 µg/mL for DPPH, and from 116.76 ± 1.31 µg/mL to 286.87 ± 0.25 µg/mL for ABTS. A simplex-centroid mixture design was employed to determine optimal component ratios. The best-performing DPPH mixture (43% stigmas, 35% leaves, 22% tepals) achieved an IC50 of 97.36 ± 1.23 µg/mL, closely matching the predicted 95.96 µg/mL. Similarly, the optimal ABTS blend (45% stigmas, 35% leaves, 20% tepals) reached an IC50 of 110.59 ± 6.82 µg/mL, aligning with the predicted 106.31 µg/mL. Both combinations outperformed standard antioxidants like BHT and ascorbic acid. These findings support the synergistic use of saffron by-products in food preservation, nutraceuticals, and biopharmaceuticals, offering an eco-friendly, cost-effective solution aligned with circular economy principles.

Citer ce document

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0