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Targeted sequencing of Rift Valley fever virus (RVFV) on Oxford Nanopore MinION platform v1

Article scientifique 2025 Anglais

Résumé

This protocol is adapted forRift Valley fever (RVF) samples and is based on the methods for the NEBNext ‱both for Illumina and Oxford Nanopore Technologies (ONT). The protocol utilizesamplicon sequences to generate consensus genomes of the virus. The targetedsequencing approach offers a viable alternative to generating RVF virus (RVFV)genomes without the need to culture, thereby reducing turnaround time forgenerating genomes. The protocol utilizes primer sets of overlapping sequencesfor Illumina (n = 78) and Oxford Nanopore Technologies (n = 258) sequencing.

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Juma, J., Konongoi, S., Kimenji, R., Muli, C., Dobi, P., Osiany, S., Kiritu, E., Bett, B., Oyola, S. (2025). Targeted sequencing of Rift Valley fever virus (RVFV) on Oxford Nanopore MinION platform v1. https://doi.org/10.17504/protocols.io.rm7vz6y3xgx1/v1

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Auteur(s)

John Juma Samson Konongoi Reuben Kimenji Collins Muli Paul O. Dobi Shebbar Osiany Edward Kiritu Bernard Bett Samuel O. Oyola

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