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No Evidence that Knops Blood Group Polymorphisms Affect Complement Receptor 1 Clustering on Erythrocytes

Article scientifique 2017 Anglais

Résumé

Abstract Clustering of Complement Receptor 1 (CR1) in the erythrocyte membrane is important for immune-complex transfer and clearance. CR1 contains the Knops blood group antigens, including the antithetical pairs Swain-Langley 1 and 2 (Sl1 and Sl2) and McCoy a and b (McCaand McCb), whose functional effects are unknown. We tested the hypothesis that theSlandMcCpolymorphisms might influence CR1 clustering on erythrocyte membranes. Blood samples from 125 healthy Kenyan children were analysed by immunofluorescence and confocal microscopy to determine CR1 cluster number and volume. In agreement with previous reports, CR1 cluster number and volume were positively associated with CR1 copy number (mean number of CR1 molecules per erythrocyte). Individuals with theMcCb/McCbgenotype had more clusters per cell thanMcCa/McCaindividuals. However, this association was lost when the strong effect of CR1 copy number was included in the model. No association was observed betweenSlgenotype, sickle cell genotype, α+thalassaemia genotype, gender or age and CR1 cluster number or volume. Therefore, after correction for CR1 copy number, theSlandMcCoypolymorphisms did not influence erythrocyte CR1 clustering, and the effects of the Knops polymorphisms on CR1 function remains unknown.

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Swann, O., Harrison, E., Opi, D., Nyatichi, E., Macharia, A., Uyoga, S., Williams, T., Rowe, J. (2017). No Evidence that Knops Blood Group Polymorphisms Affect Complement Receptor 1 Clustering on Erythrocytes. https://doi.org/10.1038/s41598-017-17664-9

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Auteur(s)

Olivia Swann Ewen M. Harrison D. Herbert Opi Emily Nyatichi Alexander W. Macharia Sophie Uyoga Thomas N. Williams J. Alexandra Rowe

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