Cell Line Treatment using Titanium dioxide Nanoparticles Irradiated by UV and IR Radiation

Résumé

Abstract Cancer is a disease characterized by abnormal cell growth in body tissues. In general, there are numerous different treatments for cancer, including surgery, chemotherapy, and radiation therapy. These therapies do, however, have negative impacts on healthy cells. The TIO2NPs is one of many pharmacological targets for the treatment of cancer by preventing cell division. Therefore, in this study, we investigated In vitro cytotoxic effects of TIO2 nanoparticles on the growth of HuH-7cells (human Hepatocellular cancer cell line). The UV–Vis (ultraviolet–visible) spectra and The cytotoxic effect have been studied of TIO2 nanoparticles after exposure to 250 W IR-radiation lamp and 11 W UV-radiation lamp for various times ranging from 2.5 to 20min and 0.5 to 10 hour respectively. The cytotoxic effect was evaluated by measuring the numbers of viable cells were determined by the MTT test. The relation between surviving cells and drug concentration is plotted to get the survival curve of each tumor cell line after treatment with the specified compound. The 50% inhibitory concentration (IC50), the concentration required to cause toxic effects in 50% of intact cells, was estimated from graphic plots of the dose response curve for each conc.

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