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Clarification of wastewater samples v1

Article scientifique 2025 Anglais

Résumé

This wastewater sample processing protocol by the Wastewater Genomics Syndicate at the National Institute of Communicable Diseases (NICD) is designed to enrich bacteria and viruses from the settled solids and supernatant components of wastewater, as different pathogens partition differently within this matrix. The end products are waterless solids and clarified wastewater supernatants, ready for downstream applications such as ultrafiltration, bead enrichment and nucleic acid extraction.

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Mabasa, V., Singh, N., Phalane, E., Macheke, M., Gwala, S., Mangena, T., Monamets, L., Rabotapi, L., Ndlovu, N., Els, F., Maposa, S., Rachida, S., McCarthy, K., Yousif, M. (2025). Clarification of wastewater samples v1. https://doi.org/10.17504/protocols.io.5jyl8qo99l2w/v1

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