A Panel of Circulating Non-coding RNAs in the Diagnosis and Monitoring of Therapy in Egyptian Patients with Breast Cancer

Résumé

Abstract 1) Background: Non coding RNAs (ncRNAs) have recently been identified to play a pivotal role in many diseases including breast cancer (BC). This study aims to investigate the relative quantification of long non-coding RNA (lncRNA) H19, microRNA (miR) 675-5p, 675-3p and miR-let 7 in BC patients.2) Methods: The study was performed on three groups, group 1; 30 non-intervened BC female patients about to undergo breast surgery, group 2; 30 postoperative female BC patients about to receive adjuvant anthracycline chemotherapy and group 3; 30 apparently healthy female volunteers as the control group. Plasma samples were drawn before and after the intervention in groups 1 and 2, with a single sample drawn from group 3. The relative quantification levels were compared with healthy control subjects and were related with the clinicopathological statuses of these patients.3) Results: There was a statistically significant increase in H19, miR-675-5p, miR-675-3p and miR-let 7 in the non-intervened BC patients when compared to the control group. Surgery resulted in a significant reduction in all four ncRNAs under investigation. Chemotherapy resulted in a significant increase in the level of miR-let 7. The assay discriminated normal from BC where the area under the receiver operating characteristic curve (AUC-ROC) of miR-675-3p showed the maximal AUC-ROC of 1.000. The diagnostic sensitivity and specificity was also 100% when CA 15-3 and H19 were combined.4) Conclusion: The results indicate that the panel of ncRNAs can all potentially act as novel biomarkers whether alone or combined in the diagnosis of BC.

Citer ce document

Accès au document

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0