Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR—a pilot study

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Abstract Objective Prompt diagnosis ofHelicobacter pyloriinfection is essential for proper treatment and eradication of the pathogen because prolonged infection could lead to gastric cancer. Sensitive and cost effective diagnostic methods are key to guiding treatment options that will reduce mortality. This study was aimed at detectingH. pylorifrom biopsies of peptic ulcer patients. Real-time PCR using TaqMan and EvaGreen assays targeting 16S rRNA andureAgenes were used to detectH. pyloriDNA extracted from 40 biopsy samples comprising 20 biopsies obtained from the antrum and 20 from the corpus of 20 patients undergoing endoscopy for duodenal ulcer investigation in Lagos, Nigeria. Results H. pyloriwas detected in 80% of the biopsy samples by combined cycle threshold (Ct) and melting temperature (Tm) values. MeanCtvalue forureAgene ranged from 21.40 to 37.53 and 22.71 to 35.44 for16SrRNAgene. Average melting temperatures (Tm) of 81.57 and 82.90 °C among amplicons ofureAand 16S rRNA were observed respectively.H. pyloriDNA was generally detected in biopsies collected from antrum and corpus. Real-time PCR in the diagnosis ofH. pylorican be considered a simple, low cost and efficient alternative or addition to the gold standard.

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