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Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

Article scientifique 2023 Anglais

Résumé

Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop's association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.

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Edwards, A., Njaci, I., Sarkar, A., Jiang, Z., Kaithakottil, G., Moore, C., Cheema, J., Stevenson, C., Rejzek, M., Novák, P., Vigouroux, M., Vickers, M., Wouters, R., Paajanen, P., Steuernagel, B., Moore, J., Higgins, J., Swarbreck, D., Martens, S., Kim, C., Weng, J., Mundree, S., Kilian, B., Kumar, S., Loose, M., Yant, L., Macas, J., Wang, T., Martin, C., Emmrich, P. (2023). Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus. https://doi.org/10.1038/s41467-023-36503-2

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Auteur(s)

Anne Edwards Isaac Njaci Abhimanyu Sarkar Zhouqian Jiang Gemy Kaithakottil Christopher Moore Jitender Cheema Clare E. M. Stevenson Martin Rejzek Petr Novák Marielle Vigouroux Martin Vickers Roland H. M. Wouters Pirita Paajanen Burkhard Steuernagel Jonathan D. Moore Janet Higgins David Swarbreck Stefan Martens Colin Y. Kim Jing‐Ke Weng Sagadevan Mundree Benjamin Kilian Shiv Kumar Matthew Loose Levi Yant Jir̆ı́ Macas Trevor L. Wang Cathie Martin Peter M. F. Emmrich

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