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A single cell atlas of in vitro multi-systems uncovers in vivo lineage trajectory and cell state in the human lung

Article scientifique 2023 Anglais

Résumé

Abstract We present an in-depth single cell atlas of in vitro multi-culture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single cell profiling identifies new cell states and differentiation trajectories of rare airway epithelial cell types in human distal lungs. By integrating single cell datasets of human lung tissues, we discover immune primed subsets enriched in lungs and organoids derived from patients with chronic respiratory disease. To demonstrate the full potential of our platform, we further illustrate transcriptomic responses to various respiratory virus infections in in vitro airway models. Our work constitutes a single-cell roadmap for the cellular and molecular characteristics of human primary lung cells in vitro and their relevance to human tissues in vivo.

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Kim, J., Lee, W., Lee, S., Yoon, J., Lee, D., Kim, Y., Han, Y., Kim, R., Moon, S., Park, Y., Park, K., Cha, B., Choi, J., Kim, J., Ha, N., Kim, K., Cho, S., Cho, N., Desai, T., Chung, J., Lee, J. (2023). A single cell atlas of in vitro multi-systems uncovers in vivo lineage trajectory and cell state in the human lung. https://doi.org/10.21203/rs.3.rs-2670400/v1

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Auteur(s)

Jong‐Il Kim Woochan Lee Seyoon Lee Jung-Ki Yoon Dakyung Lee Yuri Kim Yeon Bi Han Rokhyun Kim Sungji Moon Young Jun Park Kyunghyuk Park Bukyoung Cha Jaeyong Choi Juhyun Kim Na‐Young Ha Kwhanmien Kim Sukki Cho Nam‐Hyuk Cho Tushar Desai Jin-Haeng Chung Joo‐Hyeon Lee

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