Real-time PCR bacterial DNA detection v1

Résumé

The identification of bacterial species through molecular techniques is faster and more accurate than phenotypic identification. Analysis of the 16S rRNA gene can potentially be applied to identify a large number of bacteria. The use of universal primers designed for the sequence of the 16S rRNA gene, a conserved region among Eubacteria, allows for an initial approach to their identification by amplifying this region and subsequently sequencing the amplified fragment. Most of the time, identification is possible at the species level; however, in some cases, identification is only done at the genus level, thus requiring the use of specific primers for a more comprehensive identification. The primers used in this assay amplify all genera of Eubacteria, except for the genera Rickettsia, Coxiella, and Mycoplasma, as well as genera belonging to the Phyla Spirochaetes and Chlamydiae.

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