Peer Review Report For: Optimization of bioethanol production using stress-tolerant yeast strains isolated from household alcoholic beverages (Tella, Tej, and Areke) and molasses (as substrate) [version 1; peer review: 1 approved with reservations, 1 not approved]

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Background Yeast strains that are tolerant to several environmental stresses are extensively employed to produce bioethanol from sugar-rich feedstock. Methods In our previous research findings, five wild indigenous strains of multi-stress tolerance yeast were isolated from traditional fermented alcoholic beverages (Tella, Tej, and Areke) in Ethiopia to the purpose of evaluate their potential use for bioethanol using sugarcane molasses as a substrate. Result The optimal parameters for maximum ethanol production were pH of 4.5, 25°Brix, 30°C, 4.0 g/l ammonium sulfate, supplement with yeast extract, and 72 h of incubation period. Under these optimum conditions, 13.13 ± 0.08% (v/v) ethanol was obtained. The yield of hydrous and anhydrous bioethanol from fermented molasses samples were produced at 94.20 – 95.60% (v/v) and 99.05 – 99.56% (v/v), respectively. Conclusion According to this finding, Saccharomyces cerevisiae isolate MUT15F, Saccharomyces cerevisiae isolate MUT18F , and Saccharomyces cerevisiae isolate R9MU strains have been good candidates for production of industrial bioethanol.

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