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RNA-interference in Paramecium bursaria v1

Article scientifique 2022 Anglais

Résumé

RNA-interference based gene knock-down in Paramecium bursaria. This collection contains protocols for: 1) Paramecium cultures for RNAi - Filtering and preparing P. bursaria cultures for downstream RNAi 2) RNAi induction - Back-dilution, induction, and creating frozen E. coli feeding stocks for downstream RNAi 3) RNAi feeding - Daily resuspension and feeding of P. bursaria cultures 4) RNAi imaging - Imaging of P. bursaria cultures

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Jenkins, B. (2022). RNA-interference in Paramecium bursaria v1. https://doi.org/10.17504/protocols.io.8epv5jzm4l1b/v1

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Auteur(s)

Ben Jenkins

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