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Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

Article scientifique 2017 Anglais

Résumé

Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.

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Leitman, E., Palmer, C., Buus, S., Chen, F., Riddell, L., Sims, S., Klenerman, P., Sáez‐Cirión, A., Walker, B., Hess, P., Altfeld, M., Matthews, P., Goulder, P. (2017). Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities. https://doi.org/10.1371/journal.pone.0184496

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Auteur(s)

Ellen M. Leitman Christine D. Palmer Søren Buus Fabian Chen Lynn Riddell Stuart Sims Paul Klenerman Asier Sáez‐Cirión Bruce D. Walker Paul R. Hess Marcus Altfeld Philippa C. Matthews Philip Goulder

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