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CYP4F2 1347C>T and GGCX 12970C>G Polymorphisms as Determinants of Stable Warfarin Dose in Sudanese Patients of Heart Valve Replacement

Article scientifique 2022 Anglais

Résumé

Abstract Purpose This study intended to explore the contribution of CYP4F2 1347C > T and GGCX 12970C > G polymorphisms on warfarin dose requirements among Sudanese subjects. Methods A total of 136 Sudanese patients of heart valve replacement receiving stable warfarin dose were recruited for this study. Blood samples were collected; DNA was extracted using phenol chloroform method. Genotyping for CYP4F2 1347C > T and GGCX 12970C > G polymorphisms was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. The association of genotype with warfarin dose requirement was measured by Kruskal Wallis and Mann Whitney U tests. Genotype, age, gender, comorbidity and concurrent medication were tested as predictors of stable warfarin dose using univariate regression analysis. For all tests, p. values < 0.05 were considered statistically significant. Results Frequencies of CYP4F2 1347C > T genotypes were 22.8% CC, 61% CT and 16.2% TT. The frequencies of C, T alleles were 0.533, 0.466 respectively which are deviated from Hardy Weinberg equilibrium (p. value = 0.008). Regarding GGCX 12970C > G genotyping, 96.9% were CC and 3% were CG. The frequencies of C, G alleles were 0.984 and 0.015 respectively which are in accordance with Hardy Weinberg equilibrium (p. value = 0.859). Insignificant differences in the mean daily warfarin dose between different genotype groups were observed (all p. values > 0.05). None of the studied variables was significant predictor of stable warfarin dose in this study population (p. values > 0.05). Conclusion No significant contribution of CYP4F2 1347C > T and GGCX 12970C > G polymorphisms on warfarin dose requirements was observed in this study population.

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Elsadig, A., Elamin, E., Muddathir, A., Ali, M., Sherif, N. (2022). CYP4F2 1347C>T and GGCX 12970C>G Polymorphisms as Determinants of Stable Warfarin Dose in Sudanese Patients of Heart Valve Replacement. https://doi.org/10.21203/rs.3.rs-1624585/v1

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Ahmed Elhadi Elsadig Elwaleed M. Elamin Abdel Rahim Mahmoud Muddathir May Mohammed Ali NassrEldin M. A. Sherif

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