Investigations of Cu2ONPs - Orange Peels: Biosynthesis, Characterization, and Anticancer Activity on a Cytotoxic Breast Cancer Cell Line

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BACKGROUND: The study aimed to evaluate the efficacy of nanocomposites extracted from orange peels in targeting and combating MCF-7 cells. METHODS: We characterized the nanoparticles using XRD, FTIR, SEM, and GC-MS, and evaluated their cytotoxicity and activity using the MTT colorimetric assay and double staining technique. RESULTS: The obtained results showed that the nanoparticles ranged from 19 to 66 nanometers in size and contained active compounds in the range of 4,000-500 cm⁻¹. However, the XRD sizes of the crystalline nanoparticles ranged from 15 to 23 nm. Orange peel contains important compounds such as octanal, 1, 6-octadien-3-ol, 3, 7-dimethyl, and limonene. At 800 mg/ml, DPPH can act as a strong antioxidant and reduce free radicals by 78.29%. The addition of the molecules resulted in a trace amount of 3.99%, a viability of 74.671%, and a half-maximal inhibitory concentration of 43.75 g/mL for the cell line. The compatibility with the low concentration effectively repaired the damaged cells. CONCLUSIONS: We expect the present study to provide important insights into the influence of molecular structure on anti-cancer action.

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