Characterization and in vitro antibacterial activity of silver nanoparticles biosynthesized using phytophthora infestans biomass

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Abstract This study investigated the synthesis and characterization of biogenic silver nanoparticles (AgNPs) using biomass from Phytophthora infestans as a reducing and stabilizing agent. Fungal isolates from tomato, eggplant, pepper, and tobacco plants were purified and maintained on potato dextrose agar (PDA). Biomass was filtered and washed with Milli-Q deionized water, followed by the combination of 50 mL of 10 mM AgNO 3 solution with 50 mL of cell filtrate in a 250 mL Erlenmeyer flask. Characterization techniques, including UV‒Vis, PL, FTIR, BET, SEM, and XRD, were employed. UV‒Vis spectroscopy revealed a peak at a wavelength of 441.883 nm with an absorbance of 0.823 a.u. The Tauc plot indicated a direct bandgap of 2.038 eV, reflecting enhanced optical properties. SEM analysis revealed a heterogeneous distribution of spherical and sheet-like AgNPs with an average diameter of 42.05± $$\:0.05$$ nm. XRD confirmed a face-centered cubic structure, with the crystalline size decreasing from 12.03 nm (nonagitation) to 10.71 nm (agitation). Antibiotic assays demonstrated a dose-dependent response, with significant efficacy at 40 and 60 $$\:\mu\:g/ml$$ , while minimal activity was observed at 10 $$\:\mu\:g/ml$$ . These results highlight the potential of Phytophthora infestans biomass for biosynthesizing AgNPs with notable antibacterial properties.

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