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Genetic characterization of 11 microsatellite loci in Egyptian pigeons (Columba livia domestica) and their cross-species amplification in other Columbidae populations

Article scientifique 2018 Anglais

Résumé

AIM: speciesusing 11 microsatellite markers and to investigate the success of these markers amplification across another eight pigeon species. METHODS: but belonging to another eight pigeon species were genotyped. The polymerase chain reaction (PCR) products were electrophoresed on an ABI 3130xl DNA Sequencer. The basic measures of genetic diversity and phylogenetic trees were computed using bioinformatics software. RESULTS: showed a relatively high average(0.037). The pairwise Reynolds's genetic distance between the11 studied pigeon populations recorded lower values between Otatti and Austoraly (0.025) and between Morasla and Japanese feral pigeons (0.054). These results are supported by clustering pattern either by the neighbor-joining phylogenetic tree or by a Bayesian clustering of STRUCTURE with the admixture method. CONCLUSIONS: microsatellite markers among Egyptian domestic pigeons and across other pigeon species using cross-species amplification method. The information from this study should be useful for genetic characterization and for developing conservation programs of this important species.

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Ramadan, S., Dawod, A., El-Garhy, O., Nowier, A., Eltanany, M., Inoue‐Murayama, M. (2018). Genetic characterization of 11 microsatellite loci in Egyptian pigeons (Columba livia domestica) and their cross-species amplification in other Columbidae populations. https://doi.org/10.14202/vetworld.2018.497-505

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Auteur(s)

Sherif Ramadan Ahmed Dawod Osama El-Garhy Amira M. Nowier Marwa Eltanany Miho Inoue‐Murayama

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