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Protocol 2: Vacuum Filtration and DNA Extraction from Environmental Water Samples v1

Article scientifique 2026 Autre

Résumé

This protocol describes vacuum filtration of environmental water samples for microbial cell capture, followed by DNA extraction using the QIAamp DNA Mini Kit. It includes cell resuspension in PBS, DNA quantification using NanoDrop spectrophotometry and Qubit fluorometry, and storage conditions. The protocol was applied to water samples collected from flood-affected communities in Ghana.

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Kpeli, G., Agyirey-Kwakye, P., Livingstone, C., Cudjoe, L., Dotse, E., Nyarko, E., Zar, E., Nyarko-Otu, O., Agbogli, H., Glilekpeh, G., Korankye, S., Essandoh, P., Kabotso, D. (2026). Protocol 2: Vacuum Filtration and DNA Extraction from Environmental Water Samples v1. https://doi.org/10.17504/protocols.io.kqdg3mmkpl25/v1

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Auteur(s)

Grace Semabia Kpeli Prince Agyirey-Kwakye Counseller Nutifafa Livingstone Lillian Teye Cudjoe Emmanuel Edem Dotse Ebenezer Nyarko Ebenezer Zar Obed Nyarko-Otu Hubert Kwame Agbogli Godwin Glilekpeh Solomon Korankye Priscilla Essandoh Daniel Elorm Kabotso

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