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Profiling Transgenic Nitrogen Use Efficient Rice for Proximates, Minerals and Aminoacids

Article scientifique 2021 Anglais

Résumé

Abstract Nitrogen’s dual effects as a critical nutrient input in rice production and a major environmental pollutant necessitate developing approaches that enhance Nitrogen Use Efficiency (NUE). As a staple crop in Nigeria, the development and release of NUE rice for commercial purposes, represent an important milestone in addressing food and nutrition security challenges in the country. An important step in the establishment of biosafety baseline for the Oryza sativa Antiquinin 1/Hordeum vulgare Alanine Amino Transferase (OsAnt1/HvAlaAT) transgenic rice grains with enhanced NUE, is the conduct of compositional assessment as part of the weight of evidence approach, for evaluating whether there were any unanticipated consequences of their genetic modification. In this study, proximates, minerals and amino acid profiles of two OsAnt1/HvAlaAT transgenic rice events, NUE9 and NUE12 in comparison to their non-genetically modified (GM) counterpart New Rice for Africa (NERICA) 4 were determined. Here, results show that while transgenic rice grains had significantly altered levels of crude protein, crude fat, microminerals and aminoacids compared to controls, they all did not deviate from biological ranges available in literature. Transgenics had crude protein at levels 119.4 - 140.5% and Iron at levels 23% - 67.3% higher than controls respectively. Conversely, levels of fats and Manganese were 57.6 – 73.7% and approximately 25% respectively lower in transgenics in comparison to controls. With regards to aminoacid profile, 77.3% of aminoacids were significantly more abundant in at least one transgenic as compared to controls. Taken together, the results from this study provide preliminary basis for establishing a biosafety baseline that present evidence on the safety of NUE currently subjected to backcrossing events in Nigeria. Furthermore, the results suggest that pOsAnt1/HvAlaAT transgenic lines may not only be more efficient in utilizing N but may also be more nutritious in comparison to non-transgenic varieties.

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Amedu, J., Ibrahim, A., Kwaga, J., Ebegba, R., Akinbo, O., Ehirim, B., Samali, A. (2021). Profiling Transgenic Nitrogen Use Efficient Rice for Proximates, Minerals and Aminoacids. https://doi.org/10.21203/rs.3.rs-880335/v1

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Auteur(s)

Josephine Amedu Abdulrazak Ibrahim Jacob K. P. Kwaga Rufus Ebegba Olalekan Akinbo B. O. Ehirim Ayuba Samali

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