Effects of Artificial Light Spectra on Anthocyanin Accumulation in Blueberry Callus Cultures

Résumé

Based on the above data, it can be concluded that, WPM supplemented with 1.0 mg/L BAP in combination with 2.0 mg/L NAA was the best for callus induction and multiplication up to the fourth subculture in V. corymbosum L. cv. Sunt Blue Giant. Callus must be transferred to fresh medium every 20-25 days. Root explant was the best for in vitro production and accumulation of total anthocyanins, the callus formed from leaf origin was characterized by special qualitative composition of anthocyanins largely than in callus obtained from root origin. The application of light, especially red light, has been proven to be a promising approach for enhancing the production of anthocyanins in the callus culture of V. corymbosum L. cv. Sunt Blue Giant. Further studies could be performed on a large-scale for the production of anthocyanin by cell suspension culture of blueberry V. corymbosum.

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