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Production of Glucose Oxidase from Aspergillus Niger Using Sugarcane Peels as Carbon Source

Article scientifique 2025 Anglais

Résumé

The growing demand for eco-friendly and sustainable solutions in various industries has fuelled the demand for microbial enzymes.Industries are increasingly seeking enzyme-based alternatives to conventional chemical processes to meet regulatory requirements and consumer preferences.Also, microbial sources are considered more cost-effective than chemical synthesis and enzyme isolation from biological sources.This research aimed at producing Glucose Oxidase from Aspergillus niger using sugarcane peels as carbon source.Soil samples were collected randomly from a sugar cane market, at a bagasse dumping site in Donga Local Government Area, Taraba State, Nigeria, during the month of October 2023.Isolation of fungi was performed by soil dilution plate technique using potato dextrose agar (PDA) medium.All isolates were subjected to submerged fermentation in 250ml Erlenmeyer flasks.Morphological techniques and biochemical testing were used to confirm the desired strain for this study.The strain was cultured on PDA and incubated at 30°C for 72hours.The cultures were then preserved at 4°C and subcultured after every 7 days to maintain their viability.The fungi strain that produced glucose oxidase was screened with the O-dianisidine method.The optimal pH for glucose oxidase production was determined by measuring the enzyme activity at each of the following pH: 4-9.The optimal temperature for glucose oxidase activity was also determined using different temperatures ranging from 30°C to 600°C.Substrate concentration of the enzyme was studied.The effect of incubation days and protein concentration on glucose oxidase production and activity respectively were also investigated.The results obtained in this study showed that optimal conditions for glucose oxidase production appeared to be at day 5 of fermentation (1.658 μmolmin -1 ), with peak activity observed at a temperature of approximately 40°C (0.117 μmolmin-1) and a pH of 5 (0.161μmolmin -1 ).Glucose oxidase activity was found to increase proportionately with glucose concentration with the highest activity of glucose oxidase being 0.743µm/min at 10% glucose concentration.The evidence presented in this study revealed that glucose oxidase can be produced from isolates of Aspergillus niger.By carrying out the aim and objectives of this research, it was revealed that it is possible to produce mass quantity of crude glucose oxidase from Aspergillus niger using sugarcane peels as carbon source.This was achieved under the following conditions: incubation period of seven days, pH of 5, temperature of 40°C and substrate concentration of 10%.

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Mida, M., M.A, A., N.J, I., Audu, E., F.A, G., J.U, E., O.S, A., V.U, M., M.A, O., B.D, A., C.T, N., B.K, A., M.G, A., O.N, O., I.A., I., C.E, O., O.S, A., Asuquo, G., E.C, O., D.R, H., Okani, P., C.C, I., E.O, I. (2025). Production of Glucose Oxidase from Aspergillus Niger Using Sugarcane Peels as Carbon Source. https://doi.org/10.19080/aibm.2025.18.555997

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Auteur(s)

M Mida Abah M.A Idowu N.J Ephraim Akuaden Audu Gbadeyan F.A Ekele J.U Agbo O.S Michael V.U Oladosu M.A Adefemi B.D Nwali C.T Asare B.K Adetoyi M.G Oyibo O.N I.A. Nebesnykh I.A. Ogunta C.E Adetokunbo O.S G. O. Asuquo Odoh E.C Hassan D.R Precious Chizaram Okani Iheanacho C.C Ismaila E.O

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