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First phytochemical and antioxidant potential of essential oil and hydrolate of Asperula hirsuta Desf.

Article scientifique 2026 Anglais

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ABSTRACT This study presents the first chemical and biological investigation of the essential oil and hydrolate of Asperula hirsuta Desf. (Rubiaceae) collected from Batna, Algeria. The essential oil and hydrolate were analyzed using GC-MS and HPLC techniques, respectively, and their phenolic and flavonoid contents as well as antioxidant activities were evaluated. A total of 45 components were identified in the essential oil, which was found to be rich in terpenes (27.26%) and saturated hydrocarbons (25.21%). The hydrolate was dominated by caffeic acid, representing 34.71% of its phenolic content. Regarding phytochemical content, the hydrolate exhibited the highest levels, with a total phenolic content (TPC) of 61.98±1.91mg GAE/g and a total flavonoid content (TFC) of 120.22±7.92mg RE/g. The hydrolate also showed higher antioxidant activity than the essential oil, as assessed by DPPH and CUPRAC tests. Notably, this study reports the first evaluation of FRAP and CUPRAC tests for Asperula species. In conclusion, the richness in bioactive compounds of A. hirsuta, combined with its remarkable antioxidant activity, could make it a natural antioxidant agent with considerable potential in various applications.

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Boughachiche, A., Zaabat, N., Allaoua, Z., Aydar, E., Mertdinç, Z., Demircan, E., Ozçelik, B., Akkal, S. (2026). First phytochemical and antioxidant potential of essential oil and hydrolate of Asperula hirsuta Desf.. https://doi.org/10.1590/1678-4162-13771

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