Accès ouvert

Ceres Nanotrap & Dynabeads Combined Wastewater Virus Enrichment & Total Nucleic Acid Extraction v1

Article scientifique 2025 Anglais

Résumé

This protocol utilises Ceres Nanotrap beads and Dynabeads for virus enrichment from 20 mL wastewater samples. The Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52610) is then employed for total nucleic acid extraction. The purified nucleic acids are suitable for various downstream applications, such as real-time PCR, digital PCR, and next-generation sequencing. Both the enrichment and extraction processes are automated on the KingFisher Flex Purification System with a 24-deep-well head, using in-house adapted programmes from MagMAX_Wastewater_10mL_Flex24.

Citer ce document

Mabasa, V., Singh, N., Phalane, E., Gwala, S., Macheke, M., Mangena, T., Monametsi, L., Rabotapi, L., Ndlovu, N., Els, F., Maposa, S., Rachida, S., McCarthy, K., Yousif, M. (2025). Ceres Nanotrap & Dynabeads Combined Wastewater Virus Enrichment & Total Nucleic Acid Extraction v1. https://doi.org/10.17504/protocols.io.n2bvjdoo5vk5/v1

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Voir l'article sur le site de la revue

Auteur(s)

Victor Vusi Mabasa Natasha Singh Emmanuel Phalane Sipho Gwala Mokgaetji Macheke Thabo Mangena Lethabo Monametsi Lebohang Rabotapi Nkosenhle Ndlovu Fiona Els Sibonginkosi Maposa Said Rachida Kerrigan McCarthy Mukhlid Yousif

Statistiques

Consultations : 1

Téléchargements : 0