Whole genome amplification of West Nile virus lineage 2 v1

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West Nile virus is one of the most important endemic arbovirus in Hungary. For next-generation whole genome sequencing of West Nile virus lineage 2, a one-step reverse transcription PCR assay was developed. PCR amplicons can be used for further amplicon based sequencing protocols on Illumina MiSeq platform. The genome of the West Nile virus is a single-stranded, positive-sense, capped RNA of approximately 10–11 kb in length. The whole genome amplification was carried out by twelve overlapping PCR amplicons. For ampification of the whole genome primer sets were designed by Geneious Prime (version 2021.2.2) primer design tool.

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