Ficus benjamina as a Candidate for Cancer Therapy Due to its Selective Toxicity Property

Résumé

The study was conducted to examine the cytotoxicity of Ficus benjamina against Human Embryonic (HEK293T) cell lines. The leaves of Ficus benjamina were obtained and washed under running water, shade-dried to constant weight and ground into fine pounder, and extracted using chloroform. The chloroform extract was further separated into samples A, B, C, D, and E with the use of Thin Layer Chromatography (TLC). HEK293T cells were cultivated in DMEM or Dulbecco's Modified Eagle's Medium. The cells were then seeded at a density of 8 x 104 cells/mL in 96-well tissue culture plates, resulting in 4000 cells in 50 L of the medium in each well. With the use of the CellTitre-Glo 2.0 reagent, cytotoxicity was evaluated. In each well containing 50 liters of media, cells were treated by adding 50 liters of fresh media with a 2X concentration of the test sample and 2% DMSO, resulting in a final concentration of the test sample with 1% DMSO. After that, the treated cells were incubated for 48 hours at 37°C with 5% CO2. The luminescent signal generated was measured using a microplate luminometer with a 500 ms integration time per well.

Citer ce document

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0