The Hepatoprotective Effect of Bradykinin Potentiating Factoron Streptozotocin-induced Diabetic Rats

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Abstract Background Natural products are considered the most successful source of potential drug leads. Accordingly, the present study investigated the potential antidiabetic effect of the Egyptian honey bee venom fraction known as bradykinin potentiating factor (BPF) in streptozotocin-induced (STZ) diabetic rats. Materials & Methods An in vivo study was performed on fifty albino male rats that were divided into five groups. (G1): vehicle control animals, (G2): diabetic STZ-induced group, (G3): nondiabetic BPF-treated group, (G4): BPF-injected animals and post-treated with STZ, (G5): STZ-injected animals and post-treated with BPF. Plasma glucose levels and ALT, AST, C reactive protein (C-RP), apelin, and resistin gene expression in BPF-treated rats were evaluated and compared to STZ-treated diabetic rats and vehicle control rats. The plasma protein profile of the five animal groups was investigated by sodium dodecyl sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE). Results The data indicated that the STZ-treated (G2) group showed a highly significant increase in the levels of plasma glucose, ALT, and AST compared to the BPF-treated (G3, G4, G5), and nondiabetic control (G1) groups. Quantitative reverse transcription PCR (RT-qPCR) was carried out to amplify the apelin and resistin genes with an internal reference gene (18S rRNA) using a real-time PCR system. The concentrations of C-RP (28.3 kDa) and apelin (16 kDa) proteins observed by SDS-PAGE were higher than those of apelin, and resistin gene expression was revealed by RT-qPCR in STZ-treated (G2) rats compared with BPF-treated (G4, G5) and negative control (G1) rats. Conclusion The study concluded the importance of BPF, which has therapeutic and protective effects against STZ-induced diabetes complications. The hypoglycemic effect is revealed by the improvement of the biochemical and genetic markers, which may be attributed to BPF against diabetes complications.

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