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First case of human infection with Plasmodium knowlesi in Laos

Article scientifique 2018 Anglais

Résumé

Presentation of caseOn July 17, 2016, a 12-year-old Laotian boy living in Bengvilay village, Sanamxay district, Attapeu province (adjacent to Cambodia) in Laos visited Bengvilay Health Center.He manifested malaria-like symptoms and signs such as high fever (40 o C), shivering, and nausea.The patient did not have a history of traveling outside of Attapeu province, Laos.The fever started 3 days before he visited the health center.He was diagnosed as having been infected with Plasmodium vivax malaria by a Rapid Diagnostic Test (RDT) kit (Malaria Ag Pf/Pv, Standard Diagnostics, Republic of Korea) at the health center, and a 3-day course of artemether/lumefantrine was prescribed.The patient recovered after the treatment.Prior to this episode, the patient had been recorded as having 4 previous malaria episodes that were diagnosed by the RDT kit, but detailed information, such as Plasmodium species and symptoms, was not recorded.The family used insecticide-treated bednets inside their home.The family was employed in agriculturally related jobs and occasionally went to the forest to collect foods and other necessities.The patient sometimes accompanied his family to the forest.A finger-prick blood sample was collected on a filter paper (Whatman FTA Classic Cards, GE Healthcare Life Science, United Kingdom) prior to the treatment, and we implemented a molecular approach to confirm the Plasmodium species by PCR and DNA sequencing.We collected 2,698 finger-prick blood samples from malaria patients (positive by microscopy or RDT), including the 12-year-old Laotian boy, on the filter papers from 155 public health care facilities in the 5 southern provinces in Laos from October 2015 through October 2016 for detecting human infection with P. knowlesi.We examined all the blood samples by PCR. Approach Ethics statementThe research proposal was reviewed and approved by the National Ethics Committee for Health Research, Lao Ministry of Health (No. 049 NIOPH/NECHR), in 2014.Written informed consent was obtained from the patient's father prior to the interview and the collection of blood for DNA analysis of malaria.

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Iwagami, M., Nakatsu, M., Khattignavong, P., Soundala, P., Lorphachan, L., Keomalaphet, S., Xangsayalath, P., Kawai, S., Hongvanthong, B., Brey, P., Kano, S. (2018). First case of human infection with Plasmodium knowlesi in Laos. https://doi.org/10.1371/journal.pntd.0006244

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Auteur(s)

Moritoshi Iwagami Masami Nakatsu Phonepadith Khattignavong Pheovaly Soundala Lavy Lorphachan Sengdeuane Keomalaphet Phonepadith Xangsayalath Satoru Kawai Bouasy Hongvanthong Paul T. Brey Shigeyuki Kano

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