Comparative genome analysis and secondary metabolite prediction for endophytic bacterial strain retrieved from database: In silico approach for Bacillus subtilis subsp. subtilis 168T

Résumé

The secondary metabolites of endophytic bacteria are mainly attributed to nutrient acquisition, enhanced plant growth, and alleviated resistance against stress. This study intended to do in silico analysis and detection of biosynthetic gene clusters encoded for secondary metabolites in the complete genome of B. subtilis subsp. subtilis 168T to understand biotechnological applications. B. subtilis subsp. subtilis 168T and related strains were re-annotated using RAST and SEED web servers. AntiSMASH analysis revealed the core biosynthetic gene clusters in the genome of B. subtilis subsp. subtilis str. 168T involved in various secondary metabolite productions, including surfactin, bacillibactin, bacillaene, plipastatin, terpene, and others. From OrthoANI analysis, 91.44% genome similarity was detected between B. subtilis subsp. subtilis str. 168T and B. tequilensis KCTC 13622T. Moreover, a computational prediction of biosynthetic gene clusters was observed by protein–protein network maps for various detected secondary metabolites in the recent study. In conclusion, an endophytic bacterium, specifically B. subtilis subsp. subtilis str. 168T, was a considerable source of secondary metabolites with biocontrol and growth-promoting properties and profiles of antibiotics resistance genes due to classes of various drugs.

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