Isolation and identification of bacteria causing mastitis in small ruminants and their susceptibility to antibiotics, honey, essential oils, and plant extracts
Résumé
AIM: The present work aims to isolate and identify bacteria that cause mastitis in small ruminants and evaluates the antibacterial activity of some antibiotics, honey, essential oils, and plant extracts. MATERIALS AND METHODS: A total of 289 milk samples were collected from udder secretions of sheep (n=189) and goat (n=100) from El-Fayoum, Beni-Suef, and Giza governorates. Screening subclinical mastitis (SCM) was done using California Mastitis Test (CMT); identification of the isolates was achieved using Gram's staining, hemolytic pattern, colony morphology, and biochemical tests using Analytical Profile Index. RESULTS: . The highest bacterial resistance was found in EC (67.14%) followed by Kp (45.28%) and SA (26.57%). CONCLUSION: Onion and black cumin essential oils followed by Egyptian honey showed strong antibacterial effects against multidrug-resistant bacteria. Finally, our study proved that Egyptian honey, onion, and black cumin essential oils have a marked strong antibacterial effect against bacteria isolated from small ruminant mastitis, but still further extensive studies are needed to discover the therapeutic properties of these plant extracts and honey.
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