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Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria

Article scientifique 2024 Anglais

Résumé

Abstract Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases ( bla CTX-M-15 ) and carbapenemases ( bla NDM , bla OXA-48 -like and bla KPC ), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates. We determine presence of consistently dominant clones and their relatedness to strains causing neonatal sepsis. Higher prevalence of carbapenemases is observed in Pakistan, Bangladesh, and Ethiopia, compared to other countries, and are mostly found in surfaces near the sink drain. Klebsiella pneumoniae , Enterobacter hormaechei , Acinetobacter baumannii , Serratia marcescens and Leclercia adecarboxylata are dominant; ST15 K. pneumoniae is identified from the same ward on multiple occasions suggesting clonal persistence within the same environment, and is found to be identical to isolates causing neonatal sepsis in Pakistan over similar time periods. Our data suggests persistence of dominant clones across multiple time points, highlighting the need for assessment of Infection Prevention and Control guidelines.

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Nieto, M., Sands, K., Portal, E., Thomson, K., Carvalho, M., Mathias, J., Milton, R., Dyer, C., Akpulu, C., Boostrom, I., Hogan, P., Saif, H., Ferreira, A., Hender, T., Portal, B., Andrews, R., Watkins, W., Zahra, R., Shirazi, H., Muhammad, A., Ullah, S., Jan, M., Akif, S., Iregbu, K., Modibbo, F., Uwaezuoke, S., Audu, L., Edwin, C., Yusuf, A., Adeleye, A., Mukkadas, A., Mazarati, J., Rucogoza, A., Gaju, L., Mehtar, S., Bulabula, A., Whitelaw, A., Roberts, L., Chan, G., Bekele, D., Solomon, S., Abayneh, M., Metaferia, G., BARNARDS, G., Walsh, T. (2024). Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria. https://doi.org/10.1038/s41467-024-46684-z

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Auteur(s)

Maria Nieto Kirsty Sands Edward Portal Kathryn Thomson Maria J. Carvalho Jordan Mathias Rebecca Milton Calie Dyer Chinenye Akpulu Ian Boostrom Patrick G. Hogan Habiba Saif Ana D. Sanches Ferreira Thomas Hender Barbra Portal Robert Andrews William J. Watkins Rabaab Zahra Haider Shirazi Adil Muhammad Syed Najeeb Ullah Muhammad Hilal Jan Shermeen Akif Kenneth Iregbu Fatima Modibbo Stella Uwaezuoke Lamidi Audu C. P. Edwin Ashiru H. Yusuf Adeola Adeleye Aisha Mukkadas Jean Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andre Nyandwe Hamama Bulabula Andrew Whitelaw Lauren A. Roberts Grace J Chan Delayehu Bekele Semaria Solomon Mahlet Abayneh Gesit Metaferia Group BARNARDS Timothy R. Walsh

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