Accès ouvert

Genetic Profiling of Plasmodium falciparum Antigenic Biomarkers among Asymptomatic Pregnant Women on Intermittent Preventive Treatment with Sulfadoxine-Pyrimethamine from Southwest Nigeria

Article scientifique 2023 Anglais

Résumé

Abstract Background The genetic complexity of Plasmodium falciparum is a contributory factor to the emergence of drug-resistant parasites. The WHO recommends intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP) in malaria endemic settings. This study evaluated the prevalence of the Plasmodium falciparum multidrug resistance-1 gene (mdr-1), genetic diversity of merozoite surface proteins (msp-1, msp-2) and glutamate-rich protein (glurp) among pregnant women from southwest Nigeria. Methods One hundred PCR-confirmed Plasmodium falciparum isolates, comprising visit 1 (V1) (n = 52), delivery (n = 31) and cord blood (n = 17), were randomly selected for analysis. The mdr-1 haplotypes were evaluated using restriction fragment length polymorphism (RLFP), while the msp-1, msp-2 and glurp genes were genotyped using nested PCR. Allelic frequencies, proportions and multiplicity of infection were calculated, and the p value was considered ≤ 0.05. Results The mdr-1 (N86/N86Y) combination was detected in 11.8% (V1), 61.3% (delivery) and 58.8% (cord blood) from the isolates (p ≤ 0.05). The mutant (N86Y) haplotype was detected only in cord isolates (5.9%). The allelic frequency distribution for msp-1 was 245 (K1 = 81, MAD20 = 85 and RO33 = 79), and that for msp-2 was 110, representing 43.6% (FC27) and 56.4% (3D7), respectively. While glurp expressed the least allelic frequency of 25, 84% (V1), 12% (delivery) and 4% (cord), respectively (p ≤ 0.05). msp-1 and msp-2 recorded higher MOIs than glurp. Conclusion Antigenic falciparum strains with N86Y Pfmdr-1, msp-1, msp-2, and glurp may compromise the effectiveness of IPTp-SP in southwest Nigeria. The search for newer drug formulations for IPTp may be needed.

Citer ce document

Funwei, R., Olaleye, A., Noblefather, U., Hammed, W., Elikwu, C., Akinmade, A., Okangba, C., Akinyede, A., Ojurongbe, O., Falade, C., Walker, O. (2023). Genetic Profiling of Plasmodium falciparum Antigenic Biomarkers among Asymptomatic Pregnant Women on Intermittent Preventive Treatment with Sulfadoxine-Pyrimethamine from Southwest Nigeria. https://doi.org/10.21203/rs.3.rs-3726650/v1

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Voir l'article sur le site de la revue

Auteur(s)

Roland I. Funwei Ayodele Adegbite Olaleye Uyaiabasi Noblefather Wasiu A. Hammed Charles John Elikwu Adepoju Akinmade Chika Okangba Akinwumi Akinyinka Akinyede Olusola Ojurongbe Catherine O. Falade O Walker

Statistiques

Consultations : 1

Téléchargements : 0